What does the Bible say about final separation?

Matthew 25:31-46 describes the final separation where Christ, in His glory, will gather all nations before Him and separate them as a shepherd divides sheep from goats. The sheep, representing the elect, are placed on His right, symbolizing acceptance and justification, while the goats signify the non-elect, who are cast away to eternal punishment. This separation reflects the choices made by God before the foundation of the world, underscoring the sovereign grace that determines salvation and judgment.