What does the Bible say about spiritual regeneration?

Scripture extensively discusses the doctrine of regeneration as a supernatural act of God, wherein He imparts new life to the believer through the Holy Spirit. This transformation is articulated in John 3, where Jesus speaks of being born anew, indicating that one's spiritual condition radically changes through the work of the Holy Spirit. Furthermore, 2 Corinthians 5:17 asserts that anyone in Christ is a new creation; the old has passed away; behold, the new has come. This regeneration is not of human initiative but is a result of God’s sovereign grace, turning hearts away from sin and towards faith in Christ. It is the Spirit's operation that enables one to respond to the gospel and to live a life that reflects the character of Christ.